In situ PCR

williams at MENDEL.BERKELEY.EDU williams at MENDEL.BERKELEY.EDU
Tue May 2 15:15:26 EST 1995


Hello again,
In my continuing quest for beautiful in situ results, I'm now going to try
in situ PCR. We have borrowed a machine from Perkin Elmer, and have several
of their protocols for use with mammalian cells. Could anyone who has tried
using this technique on sectioned plant tissue give me suggestions as to
optimum fixation methods etc? Also, I'd prefer to use tissue embeded in
plastic rather than parafin. Has anyone tried this? 
Thanks in advance,
mary williams
williams at mendel.berkeley.edu




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