pollen germination assay question

Dan Coury dacoury at AG.ARIZONA.EDU
Thu May 25 12:21:10 EST 1995

I'm trying to germinate pollen from some of our mutant lines using the
Thorsness et al. (Plant Cell 5:253-261 (1993)) assay, so far without success.
My guess is that the problem is not in the culture medium but in the selec-
tion of the pollen at the proper stage of development. Does anyone have
experience with pollen germination in vitro that could suggest what stage
would be the best to assay? Any suggestions or references are greatly

Thanks, Dan Coury

* Dan Coury                          *
* Department of Plant Sciences       *
* University of Arizona              *
* Tucson, AZ 85721 USA               *
* E-mail: dacoury at ag.arizona.edu     *
* Phone:  (520) 621-9701             *
* FAX:    (520) 621-7186             *

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