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postdoc opportunity, 2d posting attempt

Wayne M. Barnes, Ph.D. barnes at BIODEC.WUSTL.EDU
Wed Nov 1 11:34:19 EST 1995

          Postdoctoral Position in St. Louis

General Areas:      Technical Development for DNA Analysis


                    Plant Genetic Engineering

More Specific Areas:  Long and Accurate PCR of viral and messenger RNA, 
                      cosmid and genomic DNA, and artificial DNA.

Even More Specific Areas:  

     Help develop PCR methodology and apply it to further increase
the reliability, fidelity, and length of product when applied to
human, plant and bacterial DNA and (RT-PCR) mRNA and plant and animal
RNA viruses.  Apply LA PCR to genomic mapping of Arabidopsis.  

        Apply LA PCR to simplify the synthesis of genes encoding Bt ICP
(Bacillus thuringiensis insect control protein) with optimized codon
frequency for expression in transgenic plants.  Target crops are
mainly in the third world, so hiree must be willing to transfer
technology as necessary.  This is old science to an extent; the new
science goals include modification of the effective ICP genes in
attempts to broaden their insecticidal activity.  

     Salary:  $23-$33K, negotiable.  

     Length of appointment:  1 to 3 years, negotiable.  

     The successful applicant is likely to have molecular experience
(PCR, cloning, sequencing, computer analysis of DNA), demonstrated
technical development skills (not just be a kit user), fine motor
skills (microliter pipetting), and good communication skills
(demonstrated ability to write your own papers and/or grant
applications in English).  Other pluses would be abilities to purify
enzymes, regenerate dicots, and regenerate monocots, particularly
transgenic ones.  

     A written quiz on laboratory common sense is part of the
interview process.  

     E me back for more details and/or apply at the address below.

Wayne M. Barnes, Ph.D.             fax: 314/362-7183
Dept. Biochemistry 8231, Washington Univ. Med.School
4566 Scott Avenue            Barnes at biodec.wustl.edu
St. Louis, Missouri 63110 USA       ph: 314/362-3351

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