g2368 CAPS primer

[Kay Schneitz]

Dear Weiqin,

some primer pairs may cause problems using standard PCR conditions. 
Usually the critical things to change are the MgCl2 concentration 
and/or the pH in the buffer, to a lesser effect the template DNA 
concentration. We use the Invitrogen PCR optimizer kit. It basically 
consists of different premade buffers you can try out. We test each 
new primer pair according to the kits protocol. Often the buffer 
composition is not critical but occasionally one gets results that 
vary from no band at all, smears, additional bands to the correct 
band only depending on the buffer. It is really worthwhile trying 
out. Especially in mapping experiments where the DNA quality/amount 
may be limiting and hence the PCR buffer conditions should be 
optimal.

Hope this helps.

Regards, Kay