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Position available

Dr David F. Marshall D.F.Marshall at bham.ac.uk
Fri Nov 17 12:33:01 EST 1995

The University of Birmingham

School of Biological Sciences
Postdoctoral Research Fellow (Grade 1A) to work on: 
Molecular and cytological characterisation of meiotic genes from 
Further Particulars

This position is to work on a project funded for up to 36 months by the 
BBSRC Plant and Microbial Sciences Committee to characterise molecular 
and cytological aspects of meiosis in Arabidopsis. Under BBSRC rules the 
grant must be activated by 1 February 1996.

Meiosis is one of the most fundamental of all biological processes, 
occupying a central role in the life cycles of all sexually reproducing 
eukaryotes. We propose to investigate the molecular, biochemical and 
cytological events that control meiosis in higher plants. Although it is 
widely acknowledged that Arabidopsis is an ideal system in which to 
examine many of the biological processes that occur in higher plants, it 
has until recently proved unsuitable for cytological studies. However we 
have developed techniques that overcome these difficulties and permit the 
detailed examination of meiosis in Arabidopsis. Cytological examination of 
T-DNA tagged mutants of Arabidopsis has enabled us to identify seven 
meiotic mutants, which exhibit a number of different phenotypic 
characteristics. Genetical analysis indicates that five of these are
likely to be T-DNA tagged. The aim of the project is to rescue the nucleotide 
sequences flanking the T-DNA inserts and to use these as molecular 
probes in order to identify clones of the wild-type genes. Transformation 
experiments will be carried out to confirm that the cloned sequences 
complement the meiotic lesions. Expression and organisation of the genes 
will be determined using standard molecular techniques. To enable 
immunolocalisation of the products of the cloned genes, Abs will be raised 
against recombinant proteins produced by high level expression in a 
suitable vector system. Immunolocalisation will be carried out using a 
range of microscopical techniques.

We are seeking a skilled, highly motivated molecular biologist or 
cytologist with a keen interest in meiosis in higher plants. Some 
experience of plant tissue culture and transformation would be 
an additional asset. 
This is a collaborative project between Dr G.H. Jones 
(G.H.Jones at bham.ac.uk; tel. 44(0)121 414 5918) and Dr F.C.H. Franklin 
(F.C.H.Franklin at bham.ac.uk; tel. 44 (0)121 414 5910), University of 
Birmingham and Dr B. Mulligan, University of Nottingham. The project 
will be based in Birmingham, however as Dr Mulligan is supervising the 
genetic analysis of the meiotic mutants, regular visits to Nottingham to
discuss progress will be essential.  
Starting salary on a scale from œ13,941-œ15,986(pounds sterling) a year 
for up to 3 years, to commence not later 
than 1st February 1996.
Application forms and further particulars are available from the Director 
of Staffing Services, The University of Birmingham, Edgbaston,, 
Birmingham B15 2TT, 
Telephone: 44 (0) 121 414 6483 (24hours),
quoting the reference number S1308/95)

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