Dear Weeds lovers,
I would like to know what there is to know about "in plant" transformation
using vacuum infiltration, as I heard along the grape vine that this method
gives superior tranformation efficiencies.
I have the method that was posted to the bulletin board by Andrew Bent Jan 94.
Does anyone have any adjustments/refinements to this method.
1. What efficiencies are people generally getting?
2. what Agro strains are best etc?
3. I would also like to know if anyone is using this technique in other plant
species especially tobacco (or is the method not suitable for bigger plants?)
any protocols greatfully accepted
I will summmarize/combine all the responses and post them on the net
for everyones enjoyment.
University of Melbourne
Parkville, Vic 3052