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immunogold detection

Michel HERZOG herzog at grenet.fr
Tue Sep 26 12:46:29 EST 1995

Dear Netters,
I'm having troubles to choose between the various published procedures for
immunogold labelling of (chloroplast) protein from Arabidopsis, including 
fixation, embedding and immunogold labelling for electron microscopy. What
is the best compromise between ultrastructure preservation and
accessibility of antigenic sites in Arabidopsis tissues? I would appreciate
to receive advices, methods and/or references. 
Many thanks in advance.
Michel Herzog  

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