We use phyC to map our matants without any problem. I guess your DNA may
not pure or contaminated, otherwise check your primer. We follow exact
procedure described for CAPS.
University of Arizona
Dept. of MCB
In article <43p3m2$drb at selene.rz.uni-duesseldorf.de>, Anja Berger
<bergera at rz.uni-duesseldorf.de> wrote:
> I am trying to map with phy C but I have problems with the PCR!
> I only recieve a smear after amplification!
> So if someone knows which reactin conditions are good for amplifying
> phyC or what I can do to reduce the smear ( I have specific primers )
> I would be very happy if you could help me with this!
> Thank you very much in advance for helping me!
> Anja Berger
> e-mail adress: bergera at rz.uni-duesseldorf.de