PhyC Problems

David Mount mount at joplin.biosci.arizona.edu
Tue Sep 26 14:02:24 EST 1995


Hi, AnjA;
We use phyC to map our matants without any problem. I guess your DNA may
not pure or contaminated, otherwise check your primer. We follow exact
procedure described for CAPS.
Good luck

zongrang liu
University of Arizona 
Dept. of MCB
In article <43p3m2$drb at selene.rz.uni-duesseldorf.de>, Anja Berger
<bergera at rz.uni-duesseldorf.de> wrote:

> I am trying to map with phy C but I have problems with the PCR!
> I only recieve a smear after amplification! 
> So if someone knows which reactin conditions are good for amplifying 
> phyC or what I can do to reduce the smear ( I have specific primers )
> I would be very happy if you could help me with this!
> Thank you very much in advance for helping me!
> Yours, 
> Anja Berger
> e-mail adress: bergera at rz.uni-duesseldorf.de



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