expression in yeast

Sandy Berry-Lowe slblowe at ALF.UCCS.EDU
Fri Apr 5 10:29:42 EST 1996


Dear Arabidopsis netters,
	We want to express an arabidopsis protein in yeast. We have
been given a cloning cassette with the GAPdH promoter to clone into.
This is a constitutive promoter. Is this a good idea, or is inducible
better? Has anyone done this? We now need a shuttle vector and 
appropriate strain to put it into. Any advice from anyone who has
done this would be greatly appreciated. Please reply directly to
me at :	slblowe at alf.uccs.edu.	Thanks, Sandy Berry-Lowe



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