Methotrexate resistance

Baudouin MICHELET michelb at ROCKVAX.ROCKEFELLER.EDU
Tue Apr 9 08:10:35 EST 1996


Hi,

I'm looking for information about the use of dihydrofolate reductase (DHFR)
as a resistance marker for Arabidopsis thaliana.

Does anyone have a working protocol for root transformation using this
marker ?  I plan to transform C24 and Landsberg erecta, but information on
any ecotype could be useful. Particularly, what concentration of
methotrexate should be used during shoot regeneration from root
transformation and what concentration is best for testing seedling
resistance ?

Thank you very much.



Baudouin


Baudouin MICHELET
Laboratory of Plant Molecular Biology (Chualab)
The Rockefeller University, New York, NY 10021-6399
Fax: 212 327 8327
Phone: 212 327 8045
E-mail: michelb at rockvax.rockefeller.edu





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