Insert-size in pBI vecter?

[N.Yoshida]

Dear Netters

I would like to introduce large genomic DNA fragments(10-20kb) to Arabidopsis
mediated by Agrobacterium. 
Does anyone have experience of such long fragments introduction via pBI
vecters?
I would like to know the possibility of transfering long fragments 
using conventional pBI systems? (What is the maximum size?)

Thanks.

Nobumasa Yoshida
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YOSHIDA, Nobumasa
Bioscience Lab.,  Mitsui Petrochem.Ind.LTD
TEL: +81-8275-3-9211  FAX: +81-8275-3-8811
E-mail: m03149 at mipec.co.jp (office)  
        BYG00240 at niftyserve.or.jp (Private)
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