Source of iaaH and iaaM genes from Agrobacterium?

viv000 viv000 at
Thu Aug 8 03:07:53 EST 1996

Many thanks to those who replied to my query.....the replies and references I 
have had are as follows.......

From: "Oswaldo Costa e Silva" <FB5A111 at>
Organization:  RRZ
To: viv000 at
Date:          Wed, 7 Aug 1996 15:08:35 MEZ
Subject:       Re: Source of iaaM and iaaH genes from Agrobacterium?
Priority: normal
Dear Adam,
I cannot help you with the genes from Agrobacterium, however, I could try to 
resuscitate my old clones from Pseudomonas syringae pv. savastanoi or tell 
you where you could get those genes.  Actually, the terminology of iaaM and 
iaaH comes from Ps. savastanoi, I believe.
Let me know what you decide.

Oswaldo da Costa e Silva, Ph.D.
Institut fuer Allgemeine Botanik
Universitaet Hamburg
Ohnhorststr. 18
22609 Hamburg
F.R. Germany
Tel.:  +49-40-822 82 391
FAX:   +49-40-822 82 503
Email: FB5A111 at  
Date: Wed, 7 Aug 96 09:35:51 -0500
From: Animesh Ray <ray at>
Subject: iaaM etc
To: Adam.Vivian-Smith at
Try Elaine Tobin at UCLA. I don't have her email but you should find it in the 
data base.

Animesh Ray
Dept of Biology
University of Rochester
Rochester, NY 14627
ray at
Date: Wed, 7 Aug 1996 08:44:05 -0500
From: "Neil E. Olszewski"  <neil at>
Reply-To: "Neil E. Olszewski"  <neil at>
To: viv000 at
Subject: Auxin Genes

Dear Adam,
Many years ago I made a binary vector, pOCA17, that contains only the auxin 
biosynthesis genes from agrobacterium.  I am willing to determine if the 
strain is alive or if vector DNA exists in my collection, if you are 
interested in this construct.  

Best wishes,
Neil Olszewski
Date: Wed, 07 Aug 1996 17:44:03 +0000 (DST)
From: casper vroemen <Casper.Vroemen at>
Subject: re: Source of iaaM and iaaH genes from Agrobacterium?
To: viv000 at
X-Envelope-To: viv000 at

The iaaH gene is listed in the NCBI database under accassion number 154746.
The reference is included, it is from Klee et al PNAS 81, 1728-1732, 1984

Casper Vroemen
Date: 7 Aug 1996 17:24:50 -0500
From: "Shah, Jyoti" <jshah at ocelot.Rutgers.EDU>
Subject: iaaH and iaaM genes
To: Adam Vivian-Smith <Adam.Vivian-Smith at>
X-Envelope-To: Adam.Vivian-Smith at

Dear Adam,
Some vectors containing the iaaH and iaaM genes were made at Monsanto company,
St. Louis, USA. I dont have the detailed address. Also you could try Harry 
Klee at Univ. Florida, Hort Sci., 1143 Fifield Hall, Post Box # 110690, 
Gainesville, Florida 32611-0690. His fax # (352)846-2063 and his e-mail 
address is hjklee at
Alternatively, you could try Rob Martienssen at the Cold Spring Harbor lab, 
Cold Spring Harbor, NY 11724, USA. His fax# (516)367-8369 and e-mail address 
is martiens at
Some references:
1. Klee et al., 1987, Genes and Development, 1:86-96
2. Sundaresan et al., 1995. Genes and Development, 9: 1797-1810.

All the best.
Jyoti Shah

In article <4u9pi0$qcf at>,
   viv000 at (viv000) wrote:
My question is regarding a source of cloned iaaM and iaaH genes from wild type 
Agrobacterium tumefaciens. Would anybody know where I could find a source of 
these genes in a vector for future cloning and manipulation?

I will repost the results of any mail I recieve regarding this query back to 
this newsgroup..
>Thanks in advance....
>Adam Vivian-Smith
>Adam Vivian-Smith
>PhD Student
>University of Adelaide/CSIRO	Voice: +61 08 303 8627
>Division of Horticulture	Fax  : +61 08 303 8601
>Urrbrae, Adelaide		Email: Adam.Vivian-Smith at
>S.A., 5064

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