M. David Marks
dmarks at BIOSCI.CBS.UMN.EDU
Thu Dec 19 16:05:02 EST 1996
Regarding Arabidopsis pollen germination and storage, check out issues of AIS
In vitro germination of Arabidopsis pollen
R. DANKERT and R.B. CONTANT
IN VITRO GERMINATION AND STORAGE OF
TRINUCLEATE ARABIDOPSIS THALIANA (L.)
You can use http://genome-www.stanford.edu/Arabidopsis/ais/EAISquery.html
to find these.
In message <email@example.com> writes:
> At 1:48 AM 12/19/96, liliane sticher wrote:
> >Dear Arabinetters,
> >Does any of you know of methods to collect, store, and germinate Arabidopsis
> >Thanks for your help.
> >Liliane Sticher
> Hi Liliane,
> I am not sure of the best way to collect Arabidopsis pollen. I have
> collected a lot of Petunia pollen for germination. I found the best way is
> to harvest the entire anther (providing it is shedding pollen). Once the
> anthers have been collected, simply add your pollen germination media to
> the anthers and LIGHTLY vortex to separate pollen from the anthers. Spin
> briefly at a low speed to pellet the anthers and then pipette out the
> supernant which contains your pollen.
> I have not had much luck storing pollen. After a day or too at 4 degrees it
> seems to loose its viability. I usually collect pollen immeadiately before
> I am ready to germinate it.
> For germination media, I use the media described by the Clarke lab - it
> works well for Petunia and tobacco pollen. I am not sure if there is a
> better reference for Arabidopsis pollen. If you need the Clarke reference,
> let me know and I can get it for you.
> Hope this helps
> Joe Verica
> Penn State
> jdc6 at psu.edu
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