pollen germination

[M. David Marks]

Regarding Arabidopsis pollen germination and storage, check out  issues of AIS

Vol 05: 

In vitro germination of Arabidopsis pollen

R. DANKERT and R.B. CONTANT

and 

Vol 26: 

IN VITRO GERMINATION AND STORAGE OF
TRINUCLEATE ARABIDOPSIS THALIANA (L.)
POLLEN GRAINS

Monika Pickert


You can use http://genome-www.stanford.edu/Arabidopsis/ais/EAISquery.html
to find these.

David Marks







In message <v01540b00aedf2718ad73@[146.186.155.89]>  writes:
> At 1:48 AM 12/19/96, liliane sticher wrote:
> >Dear Arabinetters,
> >
> >Does any of you know of methods to collect, store, and germinate Arabidopsis
> >pollen.
> >Thanks for your help.
> >
> >Liliane Sticher
> 
> 
> Hi Liliane,
> 
> I am not sure of the best way to collect Arabidopsis pollen. I have
> collected a lot of Petunia pollen for germination. I found the best way is
> to harvest the entire anther (providing it is shedding pollen). Once the
> anthers have been collected, simply add your pollen germination media to
> the anthers and LIGHTLY vortex to separate pollen from the anthers. Spin
> briefly at a low speed to pellet the anthers and then pipette out the
> supernant which contains your pollen.
> 
> I have not had much luck storing pollen. After a day or too at 4 degrees it
> seems to loose its viability. I usually collect pollen immeadiately before
> I am ready to germinate it.
> 
> For germination media, I use the media described by the Clarke lab - it
> works well for Petunia and tobacco pollen. I am not sure if there is a
> better reference for Arabidopsis pollen. If you need the Clarke reference,
> let me know and I can get it for you.
> 
> Hope this helps
> 
> Joe Verica
> Penn State
> jdc6 at psu.edu
> 
> 
>