That is dependent on replicon the vector was biuld. pBIN19, for example, can tolerate 30 kb insertion. Zongrang Liu In article <17800.199602051841 at lenzie.cent.gla.ac.uk>, gbca60 at UDCF.GLA.AC.UK (Geeta Fuglevand) wrote: > Hi, > What is the maximum length of DNA which can be integrated into a binary > vector for transformation of Arabidopsis? > Thanks in advance, > Geeta