We are planning to express an Arabidopsis protein in E. coli, with the purpose
of isolating enough protein to make antibodies against it.
We are considering to order the pET 29 vectors from Novagen, which make use of
the S-tag system. Has anyone had successful experience with this
expression/purification system? And with other vectors from the pET line?
Janette P. Fett
Department of Biological Sciences
e-mail: Janette.P.Fett at Dartmouth.EDU