Help with Differential Display

[Oswaldo Costa e Silva]

Dear Netters:
We have been working for a while now with DD in our lab.  We struggled at 
the beginning to set up the technique but are confident now that we can 
reproducibly show, through DD, differences in gene expression in our system 
(we are working with a particular maize mutant in the anthocyanin 
biosynthetic pathway).
However, we have not been able to show differential accumulation of the 
corresponding mRNA's to our cloned fragments.  We have used Northern blots 
as well as 32P labelled cDNA's to probe Southern blots of the cloned 
fragments; a technique which is advantageous considering the large number of 
differentially amplified bands.
I would be very grateful, if anyone could give us some ideas and suggestions 
on how to solve our problem.
I will post a summary of the answers.
Thanx.
Sincerely,
Oswaldo



_________________________________________________________________________
Oswaldo da Costa e Silva, Ph.D.

Institut fuer Allgemeine Botanik
Universitaet Hamburg
Ohnhorststr. 18
22609 Hamburg
F.R. Germany

Tel.:  +49-40-822 82 391
FAX:   +49-40-822 82 503
Email: fb5a111 at rrz-cip-1.rrz.uni-hamburg.de