cdt2 at PO.CWRU.EDU
Thu Feb 22 15:08:58 EST 1996
Last week I did EMS and DEB mutageneses.
This week the EMS seeds are growing fine, but nothing from the DEB treatment.
I used 1,2,3,4-Diepoxybutane (= 1,3-Butadiene epoxide) from Aldrich.
Seed imbibed overnight in the cold were treated for 4 hr at room temp in
22mM DEB in H2O (as per Ecker paper). Then washed and planted. No
germination after 9 days.
Any suggestions/help much appreciated.
More information about the Arab-gen