protocols for cell fractionation

[An-Ping Hsia]

I am looking for a protocol for getting crude protein extracts from 
nuclei, membrane fraction, and cytosol fraction from Arabidopsis 
tissues. The protein extracts will be used to study protein 
localization through Western blotting. So, enzyme activity is not 
concerned. I prefer to purify these fractions simutaneously from the 
same tissue materials. Could anyone point me a reference for such 
procedure? Thanks in advance!

Yiji Xia
Dept. of Zoology and Genetics
Iowa State University
Ames, IA50011
515-294-1659
yxia at iastate.edu