Primers for hygromycin phosphotransferase?

Mike Richberg mhr1 at email.unc.edu
Mon Jul 29 18:06:43 EST 1996


Does anyone have a sequence for primers that will amplify a hpt gene in
transgenic arabidopsis?  I'm trying to select for the presense of my T-DNA
in a mutant that is prone to die and I'm concerned that the hygro may be
killing it, even though it nominally contains the hpt gene under a Nos
promoter...

Thanks

-mike richberg




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