Sieve Recommendation?

Fred Lehle webmaster at arabidopsis.com
Mon Jun 17 12:18:38 EST 1996


We use the following two brass sieves and a bottom pan for hand
cleaning of Arabidopsis seeds. 
 
Empty siliques are removed with a 355 micrometer testing sieve
(No. 45 A.S.T.M.E-11 spec., Tyler equivalent 42 mesh) and fines
can be removed from retained seeds with a 180 micrometer
(No. 80 A.S.T.M.E-11 spec., Tyler equivalent 80 mesh) testing sieve.
These items are usually found under the category "sieve" in the 
scientific supply catalogs. 

Seed cleaning tip:  Harvest Arabidopsis plants before their stems
become completely dried out, i.e. before all green coloring has faded.

Reason:  When completely air-dried, Arabidopsis stem and leaf
tissue is extremely brittle and crumbles easily to fragments about the
same size as the seeds themselves, making them harder to clean by
sieving. 

Fred Lehle, Ph.D.
LEHLE SEEDS
webmaster at arabidopsis.com
http://www.arabidopsis.com 

russell at dogwood.botany.uga.edu (Russell L. Malmberg) wrote:

>Can anyone recommend a particular seed siever set,
>or standard mesh size screen?
>(for harvesting Arabidopsis seeds).

>Thanks,

>Russell

>********************************
> Russell L. Malmberg
> russell at dogwood.botany.uga.edu
>********************************






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