Cosmid-Vector

Toni Schaeffner schaeff at lmb.uni-muenchen.de
Wed Mar 13 12:51:15 EST 1996


Dear Arabidopsis netters,

I am seeking advice which cosmid vector out there might be best suited for
subcloning a YAC into smaller pieces which can be directly used for A.th.
transformation/ complementation.

I am aware of pBIC20 (Erwin Grill)
           of pOCA
           of pLZO3 (Bob Ludwig)

(However I did not check the availability of pOCA and pLZO3)

Parameters of particular interest to my considerations are:

stability
size range of inserts
selection in planta
availability (stock center, individual labs)


I ll post a suumary of replies.

Thank you very much,

Tony Schaeffner




*   Dr. Tony Schaeffner
*   Institut f. Biochemie
*   Ludwig-Maximilians-Universitaet
*   Wuermtalstrasse 221
*   D-81375 Muenchen, Germany
*   Tel. (0)89-7401-7311
*   Fax. (0)89-7401-7314
*   email: schaeff at lmb.uni-muenchen.de





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