Isolated DNA from pools of T-DNA lines from ABRC

Randy Scholl scholl at ABRC1.BIOSCI.OHIO-STATE.EDU
Fri Nov 1 16:57:39 EST 1996


Isolated DNA from Feldmann T-DNA Pools Now Available From ABRC

The ABRC is pleased to announce that pooled DNA from 6000 of the Feldmann
T-DNA lines is now available for PCR screening.  Ken Feldmann has recently
donated isolated DNA from his T-DNA lines as well as seeds from the
individual lines.

The distribution of the DNA will be organized in the following manner. 
Initially, six DNA samples will be sent which correspond to 6 pools of 1000
lines each.  These can be assayed by PCR and followup hybridization to
determine if a particular clone corresponds to a T-DNA insertion location.

Upon verification by the investigator, via PCR and subsequent Southern
analysis, that an insertion actually exists in a particular gene, DNA pools
from 100 lines for the appropriate 1000 lines will be sent.  These pools
for the set of 1000 lines form a two-dimensional pooling scheme (i.e.,
actually 20 samples of DNA for each set of 1000 lines) such that the
screening of row and column DNA pools should locate the insertion to a set
of 10 lines.  

The Center possesses the majority of the individual T-DNA lines.  Hence,
the investigator may then order the appropriate 10 lines identified by
screening of the 100-pools, isolate DNA from these and identify the mutant
line.

The stock number for the set of 6 1000-pools is CD5-7.  The DNA quantity
included in these samples is appropriate for at least 10 PCR reactions. 
Should individual samples need to be ordered, the stock numbers are:

	CD5-1...........pool of 1st 1000 lines
	CD5-2...........pool of 2nd 1000 lines
	CD5-3...........pool of 3rd 1000 lines
	CD5-4...........pool of 4th 1000 lines
	CD5-5...........pool of 5th 1000 lines
	CD5-6...........pool of 6th 1000 lines

Feel free to contact us with questions about these stocks.  This item may
be ordered by email (arabidopsis+ at osu.edu) or on AIMS
(http://aims.cps.msu.edu/aims/).  We consider this stock release to be a
trial for other possible sets of DNAs.  We hope that you find this new type
of stock useful.


Sincerely,

Randy Scholl
Keith Davis
ABRC





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