Isolated DNA from Feldmann T-DNA Pools Now Available From ABRC
The ABRC is pleased to announce that pooled DNA from 6000 of the Feldmann
T-DNA lines is now available for PCR screening. Ken Feldmann has recently
donated isolated DNA from his T-DNA lines as well as seeds from the
The distribution of the DNA will be organized in the following manner.
Initially, six DNA samples will be sent which correspond to 6 pools of 1000
lines each. These can be assayed by PCR and followup hybridization to
determine if a particular clone corresponds to a T-DNA insertion location.
Upon verification by the investigator, via PCR and subsequent Southern
analysis, that an insertion actually exists in a particular gene, DNA pools
from 100 lines for the appropriate 1000 lines will be sent. These pools
for the set of 1000 lines form a two-dimensional pooling scheme (i.e.,
actually 20 samples of DNA for each set of 1000 lines) such that the
screening of row and column DNA pools should locate the insertion to a set
of 10 lines.
The Center possesses the majority of the individual T-DNA lines. Hence,
the investigator may then order the appropriate 10 lines identified by
screening of the 100-pools, isolate DNA from these and identify the mutant
The stock number for the set of 6 1000-pools is CD5-7. The DNA quantity
included in these samples is appropriate for at least 10 PCR reactions.
Should individual samples need to be ordered, the stock numbers are:
CD5-1...........pool of 1st 1000 lines
CD5-2...........pool of 2nd 1000 lines
CD5-3...........pool of 3rd 1000 lines
CD5-4...........pool of 4th 1000 lines
CD5-5...........pool of 5th 1000 lines
CD5-6...........pool of 6th 1000 lines
Feel free to contact us with questions about these stocks. This item may
be ordered by email (arabidopsis+ at osu.edu) or on AIMS
(http://aims.cps.msu.edu/aims/). We consider this stock release to be a
trial for other possible sets of DNAs. We hope that you find this new type
of stock useful.