is there anyone out there who has tried the TOPP cells sold by
STRATAGENE for overexpression of difficult-to-express proteins? Any
comments are appreciated to get an idea if it is worth testing them.
I am working on a protein that I cannot get expressed in E. coli K12. What
would you recommend me to do? Should I directly switch to yeast or
I am using the pEXP expression vector and have tried different strains
of E.coli K12 for overexpression (M15 which comes with the pQE expression system from
Qiagen, W3110 as well as DH5 alpha). The correct cloning was
confirmed by sequencing through the cloning site. Except for W3110
the strains stopped growing after induction with IPTG.
I am thankful for any suggestions