I am trying to map some genes using Col and Ler.
I would like to find RFLPs between these ecotypes efficiently.
Could anyone have answer or comment to following questions?
I would appreciate any replys.
1.How often can you find RFLPs when you test a few enzymes(6 bases cutter)
for a probe(1-10kb).
I would like to ask the same question regarding CAPS(0.5-2kb) with 4-5
bases cutter enzymes
2.When I can not obtain any RFLPs with a few enzeymes, I try more enzymes
or longer prebe. Any other strategies can be taken?
Bioscience Laboratory, Mitsui Petrochem.Ind.LTD
E-mail:yoshida at mipec.co.jp