cell suspension culs

anastaso at nefeli.imbb.forth.gr anastaso at nefeli.imbb.forth.gr
Sat Nov 30 16:34:04 EST 1996

Dear netters,
we are trying to establish tobacco cell suspension
cultures using leaf callus as our starting material.
Despite our efforts using various nutrient media
(e.g. B5, MS, LS) growth (including agitation)conditions
we always come out facing the same problem. The callus
although it grows, doesnt produce individual cells even
even after having been transfered into fresh medium.
Can anyone help us overcome that problem?
Thank you

Elias Anastassopoulos

email:anastaso at nefeli.imbb.forth.gr

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