Does anyone have a protocol/reference for enriching T-DNA tagged lines for
T-DNA? Screening 7000 T-DNA tagged lines (Feldmann collection) by PCR would
be much easier if I could eliminate extraneous DNA from my genomic DNA preps.
My only lead is on a biotin/streptavadin system, where I would bind a
portion of the T-DNA gene to denatured genomic DNA containing the T-DNA tags.
This would then be coupled to streptavadin beads, and purified. If anyone
has any comments, or have found a better way to enrich T-DNA tagged lines,