BASTA for root transformation ?

Fabien Nogue fabien at PICAN.PI.CSIRO.AU
Thu Oct 24 00:21:32 EST 1996


In relation to Todd's question :

We have tried to transform C24 arabidopsis by the root transformation
technique and still have no regenerants with BASTA as a selectable marker
(The plants that we want to transform are kanamycin resistant and sterile so
we can not use the infiltration technique). Has anyone been successful in
using BASTA for root transformation? If so, what was the BASTA concentration
used in the shoot inducing media?
Thanks,
Fabien

 ~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
 ~  Fabien Nogue                                                      ~
 ~  CSIRO Division of Plant Industry   Tel:61-6-2465424               ~ 
 ~  GPO Box 1600 Canberra  ACT 2601    Fax:61-6-2465000               ~  
 ~  AUSTRALIA                          email:fabien at pican.pi.csiro.au ~
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