new stocks from ABRC
Randy Scholl
scholl at ABRC1.BIOSCI.OHIO-STATE.EDU
Wed Oct 30 16:11:06 EST 1996
Transposon seed stocks, Mi mapping and Green Fluorescent Protein clones now
available from ABRC
Several important new stocks, which are described below, are now available
from ABRC.
1. Mapped DS transposon and Ac transposase lines from the N. Fedoroff
laboratory.
(D. Smith, Y.-G. Liu, Y. Yanai, S. Ishiguro, K. Okada,
D. Shibata, R.F. Whittier and N. V. Fedoroff.
Plant J. 10:721-732)
Twenty-three new Ds transposon and five new Ac tranposase lines are
available. Ten of the Ds insertions are mapped. Crosses between the Ds
transposon and Ac transposase lines result in plants with transpositions.
Plants for which these events have occurred can then be selected. These
stocks, along with the already available mapped Ds lines from B. Baker and
C. Corr (CS8112-CS8136) and the enhancer and gene trap lines from V.
Sundaresan and R. Martienssen (CS8043-CS8049) now provide a dramatically
increased array of transposon tools available for
insertional mutagenesis. Consult the AIMS seed search page, http://aims.cps.msu.edu/aims/menu/search1.html ("new seed stocks" item) for
details of the stocks. Links from the database stock descriptions to the
Fedoroff laboratory web pages describing the use of these lines are
provided. The stock numbers of these lines are listed below along with the
chromosome number of the mapped insertions.
Stock no. donor no. chrom. no.
CS8509 Ds1 388-3
CS8510 Ds1 388-4
CS8511 Ds1 388-5 1
CS8512 Ds1 388-30 5
CS8513 Ds1 388-31
CS8514 Ds2 389-2 1
CS8515 Ds2 389-5
CS8516 Ds2 389-7
CS8517 Ds2 389-12
CS8518 Ds2 389-13 5
CS8519 Ds2 389-14 5
CS8520 Ds2 389-25 4
CS8521 Ds3 390-1 1
CS8522 Ds4 391-20 5
CS8523 Ds5 392-9
CS8524 Ds5 392-12 1
CS8525 Ds5 392-13 5
CS8526 Ds5 392-24
CS8527 Ds6 393-3
CS8528 Ds6 393-9
CS8529 Ds6 393-19
CS8530 Ds6 393-21
CS8531 Ds6 398-5
CS8532 Ds4 391-18
CS8533 BamAc 364-22-1
CS8534 BamAc 364-29-1
CS8535 BamAc 374-23-1
CS8536 NaeAc 380-6-1
CS8537 NaeAc 380-10-1
CS8538 NaeAc 380-16-1
2. Mapping clones donated by Mitsui Plant Biotechnology Research
Institute.
(Y-G. Liu, N. Mitsukawa, C. Lister, C. Dean and R. F. Whittier.
Plant J. 10:733-735)
This large new collection of plasmid mapping clones is now available for
shipping. These should be especially useful for individuals interested in
positional cloning. Consult the DNA search page,
http://aims.cps.msu.edu/aims/menu/search2.html to order these clones
and obtain information.
Stock # clone name Map position
CD3-205 mi15 1-55.7
CD3-206 mi19 1-75.9
CD3-207 mi30 4-73.1
CD3-208 mi32 4-84.5
CD3-209 mi51 4-11
CD3-210 mi54 2-55.1
CD3-211 mi61 5-101.1
CD3-212 mi62 1-55.7
CD3-213 mi63 1-74.9
CD3-214 mi69 5-122.1
CD3-215 mi70 5-123.2
CD3-216 mi72 1-81.7
CD3-217 mi74 3-1.2
CD3-218 mi79a 2-82
CD3-219 mi79b 3-70.3
CD3-220 mi83 5-97.2
CD3-221 mi87 4-42.5
CD3-222 mi90 5-36.7
CD3-223 mi97 5-13
CD3-224 mi100 1-16
CD3-225 mi103 1-137.5
CD3-226 mi106 1-98.2
CD3-227 mi107 1-55.7
CD3-228 mi111 1-55.7
CD3-229 mi112 4-81.8
CD3-230 mi116 1-56.7
CD3-231 mi121 5-8.0
CD3-232 mi122 4-14.5
CD3-233 mi123 4-100.9
CD3-234 mi125 5-60.3
CD3-235 mi128 4-69.5
CD3-236 mi133 1-79.3
CD3-237 mi137 5-69.9
CD3-238 mi138 5-32.4
CD3-239 mi139 2-34.3
CD3-240 mi142 3-38.6
CD3-241 mi148 2-35.4
CD3-242 mi157 1-152.6
CD3-243 mi163 1-52
CD3-244 mi167 4-42
CD3-245 mi172 3-3.8
CD3-246 mi174 5-21
CD3-247 mi178 3-59.6
CD3-248 mi184 5-123.2
CD3-249 mi185 1-125.8
CD3-250 mi192 1-55.7
CD3-251 mi193 1-125.8
CD3-252 mi194 5-96.2
CD3-253 mi198 4-76.1
CD3-254 mi199 3-2.2
CD3-255 mi203 1-35
CD3-256 mi204 4-13.9
CD3-257 mi207 3-18.9
CD3-258 mi208 1-97.7
CD3-260 mi219 5-44.6
CD3-261 mi225 3-48.8
CD3-262 mi230 1-109.2
CD3-263 mi232 4-102
CD3-264 mi233 4-38.4
CD3-265 mi238 2-38.1
CD3-266 mi259 1-109.2
CD3-267 mi260 4-78.2
CD3-268 mi265 1-51.4
CD3-269 mi268 3-40.3
CD3-270 mi277 2-65.7
CD3-271 mi279 4-72.6
CD3-272 mi287 3-59.6
CD3-273 mi289 3-25.4
CD3-274 mi291a 1-96.1
CD3-275 mi291b 5-67.2
CD3-276 mi301 4-23.9
CD3-277 mi303 1-106.7
CD3-278 mi304 1-109.2
CD3-279 mi306 4-39.9
CD3-280 mi310 2-17.9
CD3-281 mi320 2-9.3
CD3-282 mi322 5-26.9
CD3-283 mi323 5-79.7
CD3-284 mi324 1-114.5
CD3-285 mi328 2-9.3
CD3-286 mi330 4-81.8
CD3-287 mi334 4-39.9
CD3-288 mi335 5-143.6
CD3-289 mi339 3-25.9
CD3-290 mi342 1-76.9
CD3-291 mi348 1-30.8
CD3-292 mi353 1-114.5
CD3-293 mi355 3-9.6
CD3-294 mi357 3-10.7
CD3-295 mi358 3-69.8
CD3-296 mi369 4-139.3
CD3-297 mi372 1-10.6
CD3-298 mi386 3-47.6
CD3-299 mi390 4-25.9
CD3-300 mi398 2-28.5
CD3-301 mi403 3-9.6
CD3-302 mi408 1-109.2
CD3-303 mi413 3-67.2
CD3-304 mi418 5-123.2
CD3-305 mi421 2-18.5
CD3-306 mi422 4-92.8
CD3-307 mi423a 1-71.6
CD3-308 mi423b 5-100.5
CD3-309 mi424 1-116.2
CD3-310 mi425 1-143.4
CD3-311 mi431 4-107.5
CD3-312 mi433 5-36.2
CD3-313 mi438 5-26.9
CD3-314 mi441 1-97.2
CD3-315 mi443 1-16.6
CD3-316 mi444 2-18.5
CD3-317 mi455 2-82
CD3-318 mi456 3-101.4
CD3-319 mi462 1-136.5
CD3-320 mi465 4-66.7
CD3-321 mi467 3-13.7
CD3-322 mi473 2-83.6
3. Green fluorescent protein clones from the R. Vierstra laboratory
(S. J. Davis and R. D. Vierstra. Weeds World vol. 3.)
These useful reporter constructs are now available for distribution. The
original protein was modified for solublization as well as shift of the
spectrum of the fluorescence. The order location for these stocks is
http://aims.cps.msu.edu/aims/menu/search2.html. The three stocks are:
CD3-326 soluble-modified GFP
CD3-327 soluble-modifed and red shifted GFP
CD3-328 soluble-modified Blue FP
In addition to the above stocks, numbers of new mutants and clones have
been donated and will be available soon. Notable among these are eight
seedling/embryo mutants from U. Mayer of the G. Jurgens laboratory. The
donated mutants are gnom, gurke, fass, fackel, keule, knolle, monopterous
and knopf. Growth and characterization of these mutants is nearing
completion, and they should be available in early December. We appreciate
all of the generous stock donations of the previous year and remind you
that now is an excellent time to donate new mutants and clones.
Randy Scholl
Keith Davis
ABRC
email: arabidopsis+ at osu.edu
phone: 614-292-9371
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