We are planning to set up a yeast two-hybrid screen using the
landrace Landsberg erecta, although Columbia is being considered
as well. Is there anybody out there who has or is in the process
of making an activation-tagged library in the pJG4-5 (Gyuris et
al) vector, and would be happy to share it with us?
Alternatively, any practical tips on making such a library from
Arabidopsis are also very welcome!
John Innes Centre
Norwich NR4 7UH, UK
feys at bbsrc.ac.uk