We have come across an interesting brain teaser. One of our
colleagues has amplified a piece of DNA from a plant using a single primer
(i.e. amplified region bordered by inverted repeats...). Now the teaser
(may not be one for some of you): How can this piece of DNA be sequenced
WITHOUT having to CLONE it? In other words, the investigator wants to use
the same primers to sequence the pcr products directly after purifying
them! This conundrum has occupied parts of our brains for the last week or
so. I tend to think that this is impossible.... but then there may be some
clever way which is eluding us...
Dr. Hiranya Sankar Roychowdhury
Plant Genetic Engineering Lab.
New Mexico State University
Las Cruces, NM 88003
Ph. (505) 646-5785
hroychow at nmsu.edu