Typo in pBluescript II KS' MCS

Chun-Ming Liu liucm at osuunx.ucc.okstate.edu
Fri Aug 1 10:40:33 EST 1997


Stratagene has a pretty good reputation in most of its products.  But
this is not always true.  Today I found one "big" typo mistake in its
commonly used vector, pBluescript KS(+), in the Stratagene catalog: a 2bp
mismatch (CG-CG) within the DraII and ApaI sites (763-764) in the polylinker
region (page 310, 1997-1998 catalog).  After contacting the company,
it turns out to be a mistake in the lower strand. The right sequence should
be GC instead of CG.  Hopefully this information is useful for you sometimes.


Chun-Ming Liu, PhD

Oklahoma State University
Department of Botany
104 Life Science East
Stillwater, OK74078, USA
(1)-405-744-4336 (office)
(1)-405-744-7074 (fax)






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