Counting protoplasts

Marc Knight marc.knight at
Tue Aug 12 10:41:17 EST 1997

Dear All,

Has anyone found a good method for counting Arabidopsis protoplasts OTHER
than using a haemocytometer?  The size cut-off of the haemocytometer and
the relatively big size of the cells which makes them less 'mobile' when
they move under the coverslip makes this a poor method, in our hands at
least.  Any suggestions?

Many thanks,


Marc R Knight                       |      Department of Plant Sciences,
Tel. + 44 1865 275023               |              University of Oxford,
Fax. + 44 1865 275023               |                  South Parks Road,    |                    Oxford OX1 3RB.

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