POSTDOCTORAL POSITIONS STUDYING METAL ION TRANSPORT AND HOMEOSTASIS
Two postdoctoral positions are now available to study the molecular
biology of metal ion transport in eukaryotic cells. The current focus
of the work is the mechanism and regulation of zinc transport in the
yeast Saccharomyces cerevisiae. Related projects are also available in
both plant (Arabidopsis - joint with Mary Lou Guerinot's lab) and mammalian
Our laboratory was the first to identify a novel family of metal ion
transporters found in a diverse array of eukaryotic organisms. Further
studies have determined that the two yeast members of this family (ZRT1
and ZRT2) are responsible for zinc accumulation. These genes are
regulated at the transcriptional level in response to zinc and are
induced in zinc-limited cells. We have recently identified the
transcriptional activator (ZAP1) that is responsible, at least in part,
for this regulation. Moreover, if the intracellular pool of zinc rises
to near toxic levels, as when zinc-limited cells are suddenly exposed to
excess zinc, transporter activity is rapidly lost through a
post-translational mechanism called "feedback inactivation."
One position is funded by the U.S. Department of Energy. Potential
projects for the successful candidate include structure/function
analysis of transport proteins, the characterization of metal ion
transporter genes in mammalian cells, and an analysis of the feedback
inactivation regulatory system in yeast. A second position, funded by
the National Institutes of Health, is also anticipated and involves the
use of genetic, molecular, and biochemical approaches to study
zinc-responsive transcriptional regulation in yeast. Of particular
interest in these studies is the mechanism of metal ion sensing that
controls ZAP1 activity.
Salary will be commensurate with experience and funding for both
positions is available for up to three years. Please submit a
curriculum vitae, the names and addresses of three references, reprints
of representative publications, and a brief statement of research
interests to: David Eide, Ph.D., Nutritional Sciences Program,
Department of Biochemistry, University of Missouri-Columbia, 217 Gwynn
Hall, Columbia, MO 65211. Tel: 573-882-9686; FAX: 573-882-0185; email:
deide at showme.missouri.edu.
Zhao, H. and Eide, D. The yeast ZRT1 gene encodes the zinc transporter
protein of a high affinity uptake system induced by zinc limitation.
Proc. Natl. Acad. Sci. USA 93:2454-2458 (1996).
Eide, D., Broderius, M., Fett, J., and Guerinot, M.L. A novel,
iron-regulated metal transporter from plants identified by functional
expression in yeast. Proc. Natl. Acad. Sci. USA 93:5624-5628 (1996).
Zhao, H. and Eide, D. The ZRT2 gene encodes the low affinity zinc
transporter in Saccharomyces cerevisiae. J. Biol. Chem. 271:23203-23210
Zhao, H. and Eide, D. ZAP1: a metalloregulatory protein involved in
zinc-responsive transcriptional regulation in Saccharomyces cerevisiae.
Mol. Cell. Biol., in press (Sept. 1997).
Eide, D. Molecular biology of iron and zinc uptake in eukaryotes.
Current Opinion in Cell Biology 9: 573-577 (1997).