5,000 enhancer trap T-DNA lines now available from ABRC and NASC

daemon at net.bio.net daemon at net.bio.net
Wed Dec 17 18:19:32 EST 1997


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The Arabidopsis Biological Resource Center (ABRC) and the Nottingham
Arabidopsis Stock Centre (NASC) are pleased to announce that additional
 T-DNA lines are now available from the set donated by Dr. Thomas Jack.
 The stock numbers and prices of these are given in the stock
description provided below.  The pools of 100 lines for these are
available for immediate distribution.  The pools of 10 will be
available in early 1998, and additional lines of the 11,000+ total
population will be available around March, 1998.


We are very interested in receiving new T-DNA, transposon and mutant
stock donations.  The insertion lines are in high demand by many
laboratories, and there are many published mutant lines which we would
look forward to receiving.


The information for ordering and stock donation at NASC and ABRC are:


...............NASC............................ABRC


Web:. http://nasc.nott.ac.uk ...... http://aims.cps.msu.edu/aims

Email.arabidopsis at nottingham.ac.uk..arabidopsis+@osu.edu

Phone.+44 (0)115 9791216............+01 614-292-9371

=46ax...+44 (0)115 9513251............+01 614-292-0603

Mail..Dept of Life Science..........The Ohio State Univ.

........Nottingham University.........309 B&Z Bldg.

........Nottingham....................1735 Neil Ave.

........NG7 2RD.......................Columbus, OH 43210

........UK............................USA



---------------------------------------------



Agrobacterium transformant pools of enhancer trap lines


            from Dr. Thomas Jack Laboratory


The Thomas Jack laboratory, Dartmouth College, New Hampshire, has
donated T-DNA lines and pools which were produced in a glabrous
Columbia background (Col-6, gl1-1). A modified vacuum infiltration
procedure was utilized to generate this material. The populations were
transformed with the pD991 vector which contains an enhancer trap
construct and a constitutively expressed NPTII/kanamycin resistance
gene between the T-DNA left and right borders. The enhancer-trap
construction is comprised of a bacterial uidA encoding beta
glucuronidase (GUS) flanked by -60 minimal Cauliflower Mosaic Virus
(CaMV) promoter segment and the 3' untranslated region of the nopaline
synthase gene (3' NOS).


The pD991 vector carrying the T-DNA enhancer trap was constructed by
cloning an Asp 718/BamHI fragment (carrying the -60 CaMV/GUS fusion)
from plasmid pD979 into Agrobacterium transformation vector pCGN1547.


The distributed T-DNA pools are T4 seeds produced as follows. T1 plants
were vacuum infiltrated with the Agrobacterium culture, and the plants
grown to maturity. The kanamycin resistant T2 seeds were grown, 10 per
pot, to maturity. Each resulting pool of T3 seeds was harvested as a
group and transmitted to ABRC. The only T-DNA lines which were withheld
from the donation were a small number having specific GUS patterns in
the inflorescence. No other selections were conducted. Populations
consisting of at least 400 T3 plants for each pool of 10 lines were
grown at ABRC, and the resulting T4 pools of seeds were harvested and
maintained as a single stock.


The seed pools of these lines are distributed currently as pools of 100
plants, generated by combining the seeds from the above pools (i.e., 10
pools of 10 lines produce a single pool of 100).



STOCK #.....|.DESCRIPTION.........................|..PRICE

.............|.....................................|(ABRC-NASC)


CS/N19651*..|.50 pools of 100, all available lines|$60 - =A340



CS/N19651B**|.37 newly available pools of 100.....|$40





CS/N8155, Col-6, Columbia,glabra parental line is included in these
stocks.


*CS/N19651 are considered the currently active stocks for the Jack
lines.


** CS19651B is made available because many individuals have already
screened the previously available (from ABRC)

CS19651A set and may only need the new set of pools.



NOTE that additional pools from this donation will be made available
during 1998 and will be announced on the Arabidopsis newsgroup.  The
pools of 10 will be distributed when sufficient numbers can be
aliquoted. The individual lines for approximately the first 2000 lines
are possessed by ABRC, and are available on a limited basis, by special
arrangement and fee.</bigger></bigger></fontfamily>



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