I'm looking for information on the sensitivity of histochemical
staining for GUS ( beta-glucuronidase) activity using the substrate
X-gluc. I've been staining transgenic Arabidopsis tissue, primarily whole
leaves and floral meristems. The transgenic plants contain a translational
fusion of GUS and a putative promoter fragment. This promoter drives
expression that is readily detectable by Northern analysis- should I be
able to detect GUS expression from this promoter using X-gluc?
Thankyou in advance for helping me with this query.
Fern Ashton. (Graduate student)
E-mail: f.ashton at auckland.ac.nz