I want to transform Nicotiana pumbaginifola protoplasts with m-gfp-5ER.
Has any one transformed m-gfp5-ER in Nicotiana? Is m-GFP5-ER stable
enough for the fluorometric estimation in extracts prepared from the
protoplasts or do I need to quantitate gfp in intact protoplasts. For a
fluorometric assay either in intact protoplasts or in the protoplast
extracts how can I avoid interference from chloroplasts? m-gfp5-ER has
two excitation peaks at 400 and 473 nm and its emission peak is around
510nm (I would be grateful if someone tells me how much is it exactly).
Please e-mail me to
Ranjan.Swarup at ens-lyon.fr