I have the same problem with nga 1139 on ecotypes Col-0 and Po-1. So far I
have tried different
Mg2+ concentrations, different annealing temperatures, ranging from
40C to 65C, different buffers, differnt Taqs, different template
concentrations and preparations, Hot Start, Touchdown - in short I have
thrown everything in the book at it with no success. If anyone has been
able to get any amplification at all with this primer pair please post the
components and conditions of your witch's brew on the talk group.
banerje1 at uiuc.edu
On 20 Feb 1997, Silva, Herman P wrote:
> Hi everyone:
>> I would like to know if somebody has detected polymorphism between Ws and Col
> within the SAUR locus on bottom of chromosome 4. My second question is
> regardless of SSLP markers, I have been trying to use nga 1139 on chromosome 4
> but I can not get any amplification.
>> Any comments and/or sugestions are welcome.
>> Herman Silva