Agrobacterium Changing

Yvonne Stevens yystevens at
Wed Feb 26 18:51:17 EST 1997

Hi everyone!
        I am finding that my Agrobacterium are changing into other cells at
-80.  After triparental mating, they are testing positive for
Agrobacterium -after growth on lactose media and testing with Benedicts
solution (Agro turns Benedicts yellow). However, I am finding that they
do not respond positively to this test after an incubation period o a
few weeks at -80C.  Has anyone experienced this before and any ideas on
what I could do?

       Yvonne Stevens (yysteven at

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