GUS in seeds

WEHMEYER at DVORAK.BIOSCI.ARIZONA.EDU WEHMEYER at DVORAK.BIOSCI.ARIZONA.EDU
Thu Jan 16 00:22:23 EST 1997


post

Hi All,
I am looking for an embedding protocol for Arabidopsis seed
so that GUS can be look at in these seeds.  I am not sure if
the seeds need to be fixed before hand, if so any information
on fixing but yet retaining GUS activity will be appreciated.
The only reference I found used HistoResin for embedding of
tissue, but I could not find a distributor for HistoResin.
Has anyone used methylmethacylate with any success?
Thanks for your time.
Nadi Wehmeyer
Department of Biochemistry
Life Sciences South
University of Arizona
Tucson AZ 85721

wehmeyer at biosci.arizona.edu






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