PCR screening of transgenics

[John Shanklin]

We wish screen transgenics for our gene of interest using PCR from a single
At leaf and have tried Wang, Qi and Cutler's 1993 method (NAR 21:4153).
We seem to be having problems even with a pair of positive control primers
that amplify fine from plasmid DNA.  Are there other reliable protocols out
there that are currently used?

     John Shanklin
     Dept. of Biology, Bldg 463
     Brookhaven National Laboratory
     Upton, NY, 11973
     Phone:  516 344 3414
     Fax:      516 344 3407
     e-mail shanklin at bnl.gov