PCR screening of transgenics
shanklin at bnl.gov
Fri Jan 31 12:09:17 EST 1997
We wish screen transgenics for our gene of interest using PCR from a single
At leaf and have tried Wang, Qi and Cutler's 1993 method (NAR 21:4153).
We seem to be having problems even with a pair of positive control primers
that amplify fine from plasmid DNA. Are there other reliable protocols out
there that are currently used?
Dept. of Biology, Bldg 463
Brookhaven National Laboratory
Upton, NY, 11973
Phone: 516 344 3414
Fax: 516 344 3407
e-mail shanklin at bnl.gov
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