SEM : pollen, seeds, whole flowers

MELISSA SPIELMAN melissa.spielman at
Mon Jul 21 11:15:15 EST 1997

Dear Chris,
I can give you some advice on SEM of pollen in anthers. We dissected
dehisced anthers into 30% ethanol, and dehydrated them through an
ethanol series (1 x 1 hour in 50%, 70%, 90%, ethanol,  2 x 1 hour in 100%).
 Then they were dried
in hexamethyldisilazene (Polysciences) for 2 x 1 hour, air-dried,
mounted on stubs, and sputter-coated with gold to a depth of 20 nm.
There are some pictures of the results in the early July issue of
Development, Vol. 124 (13), on p. 2651.
Yours sincerely,
Melissa Spielman

More information about the Arab-gen mailing list