SEM : pollen, seeds, whole flowers
melissa.spielman at plant-sciences.oxford.ac.uk
Mon Jul 21 11:15:15 EST 1997
I can give you some advice on SEM of pollen in anthers. We dissected
dehisced anthers into 30% ethanol, and dehydrated them through an
ethanol series (1 x 1 hour in 50%, 70%, 90%, ethanol, 2 x 1 hour in 100%).
Then they were dried
in hexamethyldisilazene (Polysciences) for 2 x 1 hour, air-dried,
mounted on stubs, and sputter-coated with gold to a depth of 20 nm.
There are some pictures of the results in the early July issue of
Development, Vol. 124 (13), on p. 2651.
More information about the Arab-gen