Arabidopsis course

Jane Glazebrook glazebro at
Tue Mar 25 12:16:59 EST 1997

If you are interested in taking the Arabidopsis course at Cold Spring
Harbor this summer, it is still possible to apply, even though the official
deadline has passed.  Application information can be obtained from the Cold
Spring Harbor Web site (  Please send your
application as soon as possible.  The course description is as follows:


June 30 - July 20, 1997

Jane Glazebrook, University of Maryland
Eric Lam, Rutgers University
Robert Last, Boyce Thompson Institute/Cornell University


This course provides an intensive overview of topics in plant growth and
development, focusing on molecular genetic approaches to
understanding plant biology. It emphasizes recent results from Arabidopsis
thaliana and other model plants and provides an introduction to
current methods used in Arabidopsis research. The course also demonstrates
the use of microbial systems in plant research, including
Agrobacterium, E. coli and S. cerevisiae. It is designed for scientists
with experience in molecular techniques or in plant biology who wish to
work with Arabidopsis. The course consists of a vigorous lecture series, a
hands-on laboratory, and informal discussions. Speakers will
provide both an in-depth discussion of their own work and a review of their

Discussions of important topics in plant research will be presented by the
instructors and by invited speakers. These seminars will review plant
anatomy; plant development (including development of flowers, roots,
meristems, embryos and the epidermis); perception of light and
photomorphogenesis; responses to pathogens and to other environmental
stresses; synthesis and function of secondary metabolites and
hormones; nitrogen assimilation; unique aspects of plant cell biology
(including the plant cytoskeleton, cell wall, and chloroplasts); the
importance of transposons and Agrobacterium for manipulating plant genomes
and current approaches to genome analysis.

The laboratory sessions will provide an introduction to important
techniques currently used in Arabidopsis research. These include studies of
Arabidopsis development, mutant analysis, studies of epidermal features, in
situ detection of RNA, histochemical staining and
immunolabelling of proteins, transformation with Agrobacterium, transient
gene expression in protoplasts, expression of plant proteins in
microorganisms, detection and analysis of plant pathogens, and techniques
commonly used in genetic and physical mapping.

-Jane Glazebrook

Dr. Jane Glazebrook
Rm5128 Plant Sciences Bulding
University of Maryland
College Park, MD 20742
FAX 301-314-9075
glazebro at

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