synthetic Ara-medium without sugar

dnelson at aretha.biosci.arizona.edu dnelson at aretha.biosci.arizona.edu
Wed May 7 10:40:18 EST 1997


In article <287EE564B3E at botanik.uni-hamburg.de>, "Florian Wagner"
<fb6a009 at botanik.uni-hamburg.de> wrote:

> I am looking for a protocol how to grow Arabidopsis on a synthetic
> medium without any sugar. This is necessary because I want to infect
> Ara with a fungus which should not or only very limited grow on the
> Ara-medium. I tried half-concentrated Murashige-Skoog-medium without
> saccharose, but this is not optimal. Also, it is not possible to use
> any compost media, because the fungus grows very well in earth. I
> would be very grateful for any suggestions.
> --------------------------------------------------------------------
> Florian Wagner
> e-mail: wagner at botanik.uni-hamburg.de


Two suggestions:

Without sucrose, the plants must photosynthesize. Did you try high(er)
light conditions?  Also, if not present in your 1/2 MS, you might try to
add the vitamins (inositol, thiamine etc.)






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