? PCR with Arabidopsis leaf tissue?

Ivo Wiesner nfix at jcu.cz
Tue May 13 10:48:51 EST 1997


Dear Netters,
We have problems in obtaining band (detection of transgens) on the gel
from PCR with leaf tissue used as a template (after brief heat alkali
treatment followed by neutralization). With DNA from Boehringer plant
DNA isolation kit PCR works perfectly, but it is too expensive for
screening.

Has anybody out there reliable protocol for direct use of Arabidopsis
(leaf) tissue in PCR?

Thank you in advance for help or any pointer ahead.

Ivo Wiesner






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