Replies to the mapping of centromeres...
jbroadhvest at ucdavis.edu
Tue May 20 18:40:47 EST 1997
>>I wanted to know if the centomeres were mapped for any of the 5
>>chromosomes of arabidopsis. If so, where can I get the info ?
I want to thank everyone who shared info on this subject and here are some
of the responses that I got:
>My postdoc and I have identified the regions that function as centromeres
>in Arabidopsis, using tetrad analysis. We are using this method to walk to
>the centromeres and have mapped each of the cens to intervals of only a few
>centimorgans. We have enough recombinants to improve the location at least
>10-fold over the next few months. A poster with our most current
>centromere map data will be presented at the upcoming Madison Arabidopsis
>meeting (see Copenhaver and Preuss).
> Others have also looked at mapping centromeres in Arabidopsis: Koornneef
> used deletions in telotrisomic strains to obtain an approximate location
>of the functional centromeres on 4 of the Arabidopsis chromosomes (Genetica
>62:33-40), and Sears and Lee-Chen used cytogenetics to characterize some of
>the cens (Can. J. Genet. Cytol. 12:217-233).
> Richards, et al. showed that the cen region of chromosome 1 contains a
>repetitive element (NAR 19:3351-3357), and Maluszynska and Heslop-Harrison
>have used fluorscent in situ hybridization to localize
>centromere-associated repeats on intact chromosomes (Plant Journal
> Daphne Preuss Tel: (773) 702-1605
> University of Chicago Fax: (773) 702-9270
> Department of Molecular Genetics and Cell Biology
> 1103 E. 57th Street - EBC Room 304
> Chicago, IL 60637 dpreuss at midway.uchicago.edu
> Genetic map positions for A. thaliana centromeres have been
>determined through a number of means. In the early 1970s, M. Koornneef
>and co-workers used telocentric chromosome derivatives to determine the
>genetic window where cen1, 3 and 5 reside. More recently, the physical
>mapping efforts have located cen 2 and 4 to the gaps in the physical
>contig maps (bordered by a variety of repetitive DNA families).
>We have recently placed centromere repetitive DNA RFLP markers,
>identified by the 180 bp HindIII repeat class), on the Lister and Dean RI
>map... we are writing up a manuscript about this now. You can find the
>centromere markers on all five genetic maps designated as "EKR#"
>(the initials of the person, Elaine Round, in my lab who did the mapping).
>Pat Heslop-Harrison and colleagues at the John Innes have localized the
>180 bp HindIII repeats using FISH on chromosomes... they found that the
>probe hybed to the primary constriction of all five chromosomes
>(see Plant Journal 1(2):159-166 ).
>Department of Biology
>St. Louis, Missouri
>richards at biodec.wustl.edu
Section of Molecular and Cellular Biology
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Jbroadhvest at ucdavis.edu
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