Replies to the mapping of centromeres...

Jean Broadhvest jbroadhvest at ucdavis.edu
Tue May 20 18:40:47 EST 1997



>>I wanted to know if the centomeres were mapped for any of the 5
>>chromosomes of arabidopsis. If so, where can I get the info ?



I want to thank everyone who shared info on this subject and here are some
of the responses that I got:


>Dear Jean,
>
>My postdoc and I have identified the regions that function as centromeres
>in Arabidopsis, using tetrad analysis.  We are using this method to walk to
>the centromeres and have mapped each of the cens to intervals of only a few
>centimorgans.  We have enough recombinants to improve the location at least
>10-fold over the next few months.   A poster with our most current
>centromere map data will be presented at the upcoming Madison Arabidopsis
>meeting (see Copenhaver and Preuss).
>
> Others have also looked at mapping  centromeres in Arabidopsis:  Koornneef
> used deletions in telotrisomic strains  to obtain an approximate location
>of the functional centromeres on 4 of the Arabidopsis chromosomes (Genetica
>62:33-40), and Sears and Lee-Chen used cytogenetics to characterize some of
>the cens (Can. J. Genet. Cytol. 12:217-233).
>
> Richards, et al. showed that the cen region of chromosome 1 contains a
>repetitive element (NAR 19:3351-3357), and Maluszynska and Heslop-Harrison
>have used fluorscent in situ hybridization to localize
>centromere-associated repeats on intact chromosomes (Plant Journal
>1:159-166).
>
>Best wishes,
>
>Daphne Preuss
>
>=======================================================================
> Daphne Preuss                                 Tel: (773) 702-1605
> University of Chicago                    Fax: (773) 702-9270
> Department of Molecular Genetics and Cell Biology
> 1103 E. 57th Street - EBC Room 304
> Chicago, IL  60637             dpreuss at midway.uchicago.edu
>=======================================================================



>Jean,
>   Genetic map positions for  A. thaliana centromeres have been
>determined through a number of means.  In the early 1970s, M. Koornneef
>and co-workers used telocentric chromosome derivatives to determine the
>genetic window where cen1, 3 and 5 reside.  More recently, the physical
>mapping efforts have located cen 2 and 4 to the gaps in the physical
>contig maps (bordered by a variety of repetitive DNA families).
>We have recently placed centromere repetitive DNA RFLP markers,
>identified by the 180 bp HindIII repeat class), on the Lister and Dean RI
>map... we are writing up a manuscript about this now.  You can find the
>centromere markers on all five genetic maps designated as "EKR#"
>(the initials of the person, Elaine Round, in my lab who did the mapping).
>Pat Heslop-Harrison and colleagues at the John Innes have localized the
>180 bp HindIII repeats using FISH on chromosomes... they found that the
>probe hybed to the primary constriction of all five chromosomes
>(see Plant Journal 1(2):159-166 [1991]).
>
>
>Eric Richards
>Department of Biology
>Washington University
>St. Louis, Missouri
>richards at biodec.wustl.edu



Jean Broadhvest
Section of Molecular and Cellular Biology
Briggs Hall, Room 104
UCDavis
Davis, CA 95616-8535
Jbroadhvest at ucdavis.edu
Tel: 916 752 3111
FAX: 916 752 3085







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