PCR on arabidopsis leaves

yvind Johannessen oyvind.johannessen at bio.uio.no
Tue May 20 15:24:28 EST 1997


Dear Ivo Wiesner, we use the DNA-direct kit from Dynal. You can easily
extract enough DNA for numbers of PCR-reactions using a seedling with the
two cotelydons only. This method was published by Rudi et al. in
Biotechniques vol. 22 no. 2 1997. We also have a method were we from 40
to 80 mg tissue can get 1 to 5 microgram DNA, ok for southern and PCR.

--
Dr. Reidunn B.Aalen,
Associate Professor
Division of General Genetics
P.O.Box 1031 Blindern
N-0315 Oslo, Norway
Tlf. (+47) 22 85 44 37
Fax  (+47) 22 85 46 05
E-mail r.b.aalen at bio.uio.no






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