Transformation using kanamycin

[Gijs van Rooijen]

Dear all,

Recently we have been experiencing a dramatic reduction in the
transformation frequency of B.napus when we use kanamycin as a
selectable agent.  We are suspecting that our kanamycin is the problem.
We are suspecting that our kanamycin contains a contaminant which
severly inhibits regeneration. Has anybody out there experienced a
similar problem when using kanamycin in plant transformation?

I will summarize the responses and post them back to this newsgroup


Gijs

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