How to determine the number of T-DNA insertions at a locus

Gilles VACHON Gilles.Vachon at ujf-grenoble.fr
Mon Oct 13 13:28:10 EST 1997


Hi all,

We are facing the problem of determining the number of T-DNA insertions at
a locus. Our lines carrie a GUS gene under control of a specific promoter
and segregate 3:1 regarding Kanamycine resistance.
What are is the best strategy for doing so and avoid problems in
interpreting the results (tandem insertions etc..)? cut inside GUS? with
one enzyme, two enzymes.
Help or references are appreciated
Thanks

Gilles Vachon
Laboratoire de Genetique Moleculaire des Plantes,
UMR 5755, CERMO
Universite J. Fourier, BP 53X
38041 GRENOBLE CEDEX
FRANCE
Tel: (33) 76 63 56 58
fax: (33) 76 51 43 36
e-mail: Gilles.Vachon at ujf-grenoble.fr







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