getting ride of rubisco

Jon Monroe monroejd at jmu.edu
Mon Oct 13 19:07:47 EST 1997


Thomas Leustek wrote;

>My enzyme is of low abundance in leaves and it runs slighly below rubiscoLS
>on SDS-PAGE gels. This cuases a problem with immunoblotting...
...
> I would appreciate any advice to improve the immunoblot
>results:
>
>(1) an easy technique for selectively removing rubisco-

We've found that the rubiscoLS precipitates in the 0-10% ammonium sulfate
fraction.  If your protein precipitates at a higher percentage of ammonium
sulfate, that might do the trick.  It is a nice concentration step as well.

Jon

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