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Transformation specialist

Greg Martin gmartin at dept.agry.purdue.edu
Thu Oct 30 12:05:05 EST 1997

POSITION ANNOUNCEMENT:  Plant transformation specialist
(LABORATORY TECHNICIAN LEVEL VI), Agronomy Department, Purdue University

Serve as a plant transformation specialist to support a research program which
investigates the molecular basis of disease resistance in plants.

1) Produce transgenic tomato, tobacco and potato plants using
Agrobacterium-based transformation methods; 2) Produce and maintain plant
suspension cell cultures and callus tissue; 3) Maintain plants in the
greenhouse including sowing seeds, watering, fertilizing, making crosses,
harvesting seeds, and keeping seed inventory; and 4) Perform experimental
procedures to characterize transgenic plants including inoculation of plants
with characterized pathogens, scoring symptoms, preparation of DNA and RNA,
hybridization with radiolabeled DNA probes, and determining protein expression
with ELISA and western blots.


Education:  B.S. in genetics, plant pathology, horticulture, agronomy or
related field.

Experience:  Recent experience with Agrobacterium-mediated plant
transformation, plant tissue culture, plant maintenance, general laboratory
techniques, and some background in molecular biology.

Skills required:  Successful plant transformation requires technical skill to
perform many laboratory procedures, planning and preparation for complicated
procedures, excellent sterile technique, ability to troubleshoot when things go
wrong, meticulous attention to detail and careful record-keeping.

Knowledge of microbiology procedures including media preparation, growing
bacteria, and molecular biology procedures such as plasmid preparation,
cloning, hybridization,RNA and genomic DNA isolation, gel electrophoresis

Other: Must be reliable, have independent judgment, have initiative, and enjoy
working with people in a scientific setting.  Ability to prioritize your work,
efficiently schedule your time, and meet goals.

Pay scale:  Depends upon experience and qualifications.

Duration:  Two years.  Benefits apply.

To apply contact Purdue Personnel Services at 49-47417 (position number: 21560)
For more information contact Dr. Greg Martin (4-4790) Department of Agronomy,
Purdue University, West Lafayette, IN 47907-1150, email:
gmartin at dept.agry.purdue.edu
or see web page:  http://www.adpc.purdue.edu/personnel/currjobs/newjobs.html

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